Structural property of soybean protein P34 and specific IgE response to recombinant P34 in patients with soybean allergy
نویسندگان
چکیده
منابع مشابه
Cross-Reactivity Between the Soybean Protein P34 and Bovine Caseins
PURPOSE Soy-based formulas are widely used as dairy substitutes to treat milk allergy patients. However, reactions to soy have been reported in a small proportion of patients with IgE-mediated milk allergies. The aim of this work was to explore whether P34, a mayor soybean allergen, is involved in this cross-reactivity. METHODS In vitro recognition of P34 was evaluated by immunoblotting, comp...
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Soybean ranks among the "big 8" of the most allergenic foods, and with increasing consumption of soybean products, the incidence of soy-caused allergies is expected to escalate. Soybean and its derivatives have become ubiquitous in vegetarian and many meat-based food products, and as a result, dietary avoidance has become difficult. However, soybeans can be manipulated in a variety of ways to a...
متن کاملIsolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography
BACKGROUND Soybeans play a prominent role in allergologic research due to the high incidence of allergic reactions. For detailed studies on specific proteins it is necessary to have access to a large amount of pure substance. RESULTS In this contribution, a method for purifying soybean (Glycine max) protein P34 (also called Gly m Bd 30 K or Gly m 1) using hydrophobic interaction chromatograph...
متن کاملSoybean allergy: IgE epitopes of glycinin (Gly m 6), an important soybean allergen
Methods 11 subjects with clinically confirmed soybean allergy and sIgE against rGly m 6 were enrolled in this study. Synthetic overlapping peptides (15-mers, 4 aa offset) representing the amino acid sequences of G1 and G5, two representative subunits of Gly m 6, were spotted onto CelluSpotTM slides and analyzed for IgE-binding, respectively. The sequences of IgE-binding peptides were allocated ...
متن کاملUsefulness of wheat and soybean specific IgE antibody titers for the diagnosis of food allergy.
BACKGROUND Since the first suggestion of threshold values for food specific IgE antibody levels in relation to clinical reactivity, several authors have proposed different threshold values for different allergens. We investigated the relationship between wheat/soybean specific IgE antibody levels and the outcome of wheat/soybean allergy diagnosis in children of different ages. METHODS A retro...
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ژورنال
عنوان ژورنال: International Journal of Molecular Medicine
سال: 2011
ISSN: 1107-3756,1791-244X
DOI: 10.3892/ijmm.2011.841